KMID : 0368420150580060383
|
|
Journal of Plant Biology 2015 Volume.58 No. 6 p.383 ~ p.390
|
|
Analysis of cytosine methylation status in potato by methylation-sensitive amplified polymorphisms under low-temperature stress
|
|
Xin Cuihua
Hou Ruikun Wu Fei Zhao Yibo Xiao Huanhuan Si Wantong Ali Md Emran Cai Lu Guo Jiangbo
|
|
Abstract
|
|
|
China is the leading producer of potatoes (Solanum tuberosum) in the world. However, low-temperature stress can severely restrict the yield and quality of potato crops in that country. We used methylation-sensitive amplified polymorphisms (MSAPs) to analyze how such stress might alter the status of cytosine methylation in potato cultivar ¡®Atlantic¡¯. Over 2400 loci were identified and assessed for DNA methylation in chilling-treated and control plants. The level of cytosine methylation increased from 37.71% to 56.51% after 48 h of treatment but then decreased to 42.14% after 60 h of chilling. Stress-induced changes in the banding patterns of DNA methylation primarily resulted from full methylation of CG (internal cytosine of 5¡¯-CCGG-3¡¯) on double DNA strands. We sequenced 78 polymorphic DNA fragments and blasted them against NCBI. Expression patterns of 4 genes were then assessed via semi-quantitative RT-PCR. The expression patterns of genes related to chilling stress appeared to be regulated by the methylation or demethylation status in potato.
|
|
KEYWORD
|
|
Cytosine methylation, Low-temperature stress, MSAP, Potato
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|